Related Experiment Video
Updated: Sep 4, 2025
10:38
Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
Published on: September 27, 2012
22.6K
ATP Sensing Paper with Smartphone Bioluminescence-Based Detection.
Maria Maddalena Calabretta1,2, Ruslan Alvarez-Diduk3, Elisa Michelini4,5
1Department of Chemistry "Giacomo Ciamician", University of Bologna, Bologna, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|July 14, 2022
Summary
We developed a simple ATP sensing paper using freeze-drying for easy detection of Adenosine-5'-triphosphate (ATP), the main energy molecule in cells. This method is useful for hygiene monitoring and food safety applications.
Area of Science:
- Biochemistry
- Bioanalytical Chemistry
- Food Science
Background:
- Adenosine-5'-triphosphate (ATP) is crucial for cellular energy transfer in all organisms.
- ATP detection is a standard method for assessing cellular activity and viability.
- ATP-driven bioluminescence (BL) assays are widely used for hygiene monitoring and detecting microbial contamination in food.
Purpose of the Study:
- To develop a straightforward and accessible method for Adenosine-5'-triphosphate (ATP) bioluminescence (BL) detection.
- To create an ATP sensing paper utilizing an alternative freeze-drying technique.
- To enable ATP detection using readily available laboratory equipment and portable devices.
Main Methods:
- Fabrication of ATP sensing paper via an alternative freeze-drying procedure.
- Utilizing the D-luciferin-luciferase reaction for ATP-driven bioluminescence.
- Implementing detection with standard benchtop luminometers or portable devices, including smartphone cameras.
Main Results:
- Successful fabrication of ATP sensing paper using a novel freeze-drying method.
- Demonstrated straightforward ATP BL detection capabilities.
- Compatibility with both conventional luminometers and smartphone-based detection systems.
Conclusions:
- The developed ATP sensing paper offers a simple and accessible approach for ATP detection.
- The method leverages common laboratory technologies like freeze-drying, wax printing, and 3D printing.
- This technique facilitates ATP BL detection using readily available instrumentation, including smartphones, enhancing its applicability in various settings.
