Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Corrigendum to "Revisiting subcallosal cingulate deep brain stimulation for depression: Long-term safety and effectiveness outcomes from a pooled analysis of 172 implanted patients" [Brain Stimul 18 (2025) 1632-1640].

Brain stimulation·2026

Same author

Exercise evokes retained motor performance without neuroprotection in a mouse model of Parkinson's disease.

Frontiers in physiology·2026

Same author

Gut microbiome modulation of opioid reinforcement in preclinical models: Microbial regulation of reward and motivation.

Brain research bulletin·2026

Same author

Magnetic resonance-guided focused ultrasound for the management of tremor: update on the position statement of the American Society for Stereotactic and Functional Neurosurgery.

Journal of neurosurgery·2026

Same author

Neuropsychological outcomes comparing traditional surgical approaches and laser interstitial therapy for refractory mesial temporal lobe epilepsy: A systematic review and meta-analysis.

Epilepsia·2026

Same author

Safety assessment of temporal interference stimulation.

Frontiers in neuroscience·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 4, 2025

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

Bioluminescence-Optogenetics: A Practical Guide.

Matthew A Stern¹, Henry Skelton¹, Alejandra M Fernandez¹

¹Department of Neurosurgery, Emory University School of Medicine, Atlanta, GA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 14, 2022

Summary

This summary is machine-generated.

We introduce bioluminescence-optogenetics (BL-OG), a novel method using light-emitting proteins to control neural activity. This technique offers a new avenue for neuroscience research and potential treatments for neurological disorders.

Keywords:

Bioluminescence (BL)Channelrhodopsin Chemogenetics Coelenterazine Halorhodopsin Luciferase Luciferin Luminopsins (LMOs)Optogenetics (OG)Step-function opsin (SFO)

More Related Videos

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Published on: January 12, 2021

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

Related Experiment Videos

Last Updated: Sep 4, 2025

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Published on: January 12, 2021

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

Area of Science:

Neuroscience
Molecular Biology
Optogenetics

Background:

Genetic manipulation of neural activity is crucial for neuroscience research.
It holds potential for treating neurological and psychiatric disorders.
Existing methods require external light sources.

Purpose of the Study:

To describe bioluminescence-optogenetics (BL-OG), a novel neuromodulation technique.
To provide a practical guide for applying BL-OG and luminopsins (LMOs) in vitro.
To lay the groundwork for future in vivo applications.

Main Methods:

Utilizing bioluminescence (BL) to activate light-driven ion channels and pumps in neurons.
Developing opsin-luciferase fusion proteins called luminopsins (LMOs).
Applying BL-OG in cell lines and primary neuronal cultures.

Main Results:

Demonstrated the feasibility of controlling neural activity using BL-OG in vitro.
Established luminopsins (LMOs) as effective tools for BL-OG.
Provided a foundational protocol for BL-OG implementation.

Conclusions:

Bioluminescence-optogenetics (BL-OG) offers a promising, light-independent approach for neural activity control.
Luminopsins (LMOs) are effective tools for this novel neuromodulation technique.
This in vitro study paves the way for advanced BL-OG applications in complex biological systems.