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Human trabecular meshwork organ culture. A new method.

D H Johnson, R C Tschumper

Investigative Ophthalmology & Visual Science

|June 1, 1987

Summary

A novel organ culture method preserves human trabecular meshwork structure and function. This technique enables controlled experimental study of the trabecular meshwork, crucial for understanding intraocular pressure regulation.

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Area of Science:

Ophthalmology
Cell Biology
Biomedical Engineering

Background:

The trabecular meshwork is vital for regulating intraocular pressure.
Studying the human trabecular meshwork ex vivo presents significant challenges.
Existing methods often fail to maintain the tissue's native architecture and cellular integrity.

Purpose of the Study:

To develop a new method for organ culture of intact human trabecular meshwork.
To establish a system for controlled experimental investigation of trabecular meshwork physiology.
To maintain corneal clarity and trabecular cell morphology during culture.

Main Methods:

Human eyebank eyes were sectioned, and anterior segments were placed in a modified, sealed culture dish.
Culture media was perfused through the trabecular meshwork and Schlemm's canal via a bottom cannula.
Intraocular pressure was maintained at physiological or elevated levels throughout the culture period.

Main Results:

The developed method successfully maintained corneal clarity and trabecular cell morphology.
Trabecular cells remained in their normal position on trabecular beams.
Cultures demonstrated viability for up to 4 weeks, with media flowing through normal limbal pathways.

Conclusions:

This novel organ culture technique provides a viable system for studying the intact human trabecular meshwork.
The method allows for controlled experimental manipulation of the trabecular meshwork.
This advancement facilitates research into the mechanisms of intraocular pressure regulation and related diseases.

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