Structural characterization and a novel water-soluble polysaccharide from Fomes Fomentarius

At present, there is no report on the anti-hypoxia activity of fomes fomentarius polysaccharide. Therefore, our aim was to evaluate the anti-hypoxia activity of the purified exopolysaccharide of Paragonimus fordii. The extracellular polysaccharide of Trichophyton was screened and purified by DEAE-52 chromatographic column, and the fraction was FFEP-1 (molecular weight = 3.08 × 10⁵ DA). The analysis of monosaccharide components showed that FFEP-1 was mainly constructed by galactose, mannose and glucose. The main chain of FFEP-1 is composed of β-1,2-connected GALP β-1,2,3-connected GALP α-1,3-connected GLCP β-1,3,4-linked Manp composition, and α-1,3-linked Manps are identified in O-2 and O-3 α-1-galp at the end and α-1-terminal GLCP substitution. The possible structure of FFEP-1 was determined by one-dimensional and two-dimensional NMR and methylation analyses. The anti-hypoxia effect of FFEP-1 was studied through various experiments, which showed that FFEP-1 had a similar anti-hypoxia effect on propranolol hydrochloride. The anti-hypoxia effect of FFEP-1 might be explained by increasing the number of red blood cells and hemoglobin content.