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Updated: Sep 4, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
Published on: January 26, 2024
Lightsheet Microscopy
Laura Dyer1, Andrew Parker1, Keanu Paphiti1
1MRC Harwell Institute, Mammalian Genetics Unit, Harwell Campus, Oxfordshire, UK.
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In this paper, we review lightsheet (selective plane illumination) microscopy for mouse developmental biologists. There are different means of forming the illumination sheet, and we discuss these. We explain how we introduced the lightsheet microscope economically into our core facility and present our results on fixed and living samples. We also describe methods of clearing fixed samples for three-dimensional imaging and discuss the various means of preparing samples with particular reference to mouse cilia, adipose spheroids, and cochleae. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.
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