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Related Experiment Video

Updated: Sep 4, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

Lightsheet Microscopy.

Laura Dyer¹, Andrew Parker¹, Keanu Paphiti¹

¹MRC Harwell Institute, Mammalian Genetics Unit, Harwell Campus, Oxfordshire, UK.

Current Protocols

|July 15, 2022

Summary

This summary is machine-generated.

This review covers lightsheet microscopy (selective plane illumination microscopy) for mouse developmental biology. It details economical implementation in core facilities and sample preparation for 3D imaging of cilia, spheroids, and cochleae.

Keywords:

Bessel beam Gaussian beam airy beam cilia imaging clearing cochlear imaging fluorescence microscopy lattice lightsheet lightsheet microscopy selective plane illumination microscopy spheroid imaging

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Area of Science:

Developmental Biology
Microscopy Techniques
Imaging Science

Background:

Lightsheet microscopy offers advantages for biological imaging.
Understanding its implementation and sample preparation is crucial for researchers.

Purpose of the Study:

To provide a comprehensive review of lightsheet microscopy for mouse developmental biologists.
To guide researchers on economical implementation and sample preparation for 3D imaging.

Main Methods:

Review of different illumination sheet formation methods.
Description of economical lightsheet microscope introduction into a core facility.
Methods for clearing fixed samples for 3D imaging.
Sample preparation techniques for specific mouse tissues.

Main Results:

Demonstration of lightsheet microscopy on fixed and living mouse samples.
Successful application of sample clearing and preparation techniques.
Insights into cost-effective integration of advanced microscopy.

Conclusions:

Lightsheet microscopy is a valuable tool for mouse developmental biology research.
Economical implementation and optimized sample preparation enhance 3D imaging capabilities.
The review provides practical guidance for researchers utilizing this technique.