Ascorbate oxidase enabling glucometer readout for portable detection of hydrogen peroxide

Area of Science:

• Analytical Chemistry
• Biotechnology
• Biosensors

Background:

• Accurate hydrogen peroxide (H2O2) detection is crucial in various fields.
• Existing H2O2 detection methods can be complex, costly, or lack portability.
• Personal glucose meters (PGMs) offer a user-friendly platform for electrochemical sensing.

Purpose of the Study:

• To develop a rapid, portable, and cost-effective H2O2 quantification method.
• To establish a novel biosensing approach utilizing ascorbate oxidase (AAO) and a PGM.
• To demonstrate the feasibility of PGM-based H2O2 detection for practical applications.

Main Methods:

• Utilized ascorbate oxidase (AAO) to catalyze H2O2 reactions.
• Employed a personal glucose meter (PGM) for signal detection.
• Leveraged the reaction between ascorbic acid (AA) and ferricyanide for PGM signal generation.
• Optimized experimental conditions for H2O2 detection.

Main Results:

• Established a linear detection range for H2O2 from 2.5 to 5 × 10^3 μM.
• Achieved a quantification limit of 2.5 μM for H2O2.
• Demonstrated high spiked recovery rates (95.3-108.9%) in real samples including tap water, contact lens solution, medical H2O2, and human serum.
• Confirmed the feasibility of the PGM-based method for practical H2O2 analysis.

Conclusions:

• Successfully developed a novel, PGM-based method for H2O2 quantification.
• The method is rapid, portable, cost-effective, and exhibits good accuracy and sensitivity.
• This approach offers a simple and practical alternative for H2O2 detection in diverse real-world samples.