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Related Experiment Video

Updated: Sep 4, 2025

Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging
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Protocol for culturing and imaging of ectodermal cells from Xenopus.

Nydia Tejeda-Muñoz1, Julia Monka1, Edward M De Robertis1

  • 1Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1662, USA.

STAR Protocols
|July 15, 2022
PubMed
Summary

This study presents a new protocol for culturing and imaging Xenopus cap cells. This method allows visualization of key cellular processes like Wnt signaling and cell migration in vitro.

Keywords:
Cell BiologyCell cultureDevelopmental biologyMicroscopyModel OrganismsSignal Transduction

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Background:

  • The Xenopus embryo is a valuable model for gene manipulation and studying developmental processes.
  • Understanding cellular behaviors in a controlled environment is crucial for dissecting complex biological functions.

Purpose of the Study:

  • To establish and present a detailed protocol for culturing and imaging explanted Xenopus cap cells.
  • To demonstrate the utility of this protocol for visualizing diverse cellular processes.

Main Methods:

  • Development of a tissue culture protocol for Xenopus cap cells.
  • Application of imaging techniques to observe cellular components and dynamics.
  • Utilizing gene transplantation and depletion methods within the Xenopus model.

Main Results:

  • Successful culturing and imaging of Xenopus cap cells in vitro.
  • Demonstrated visualization of lysosomes, macropinocytosis, and focal adhesions.
  • Enabled observation of Wnt signaling pathways and cell migration dynamics.

Conclusions:

  • The presented protocol provides a robust method for studying Xenopus cap cell biology.
  • This technique facilitates in-depth analysis of fundamental cellular processes in a tractable model system.