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Updated: Sep 4, 2025

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
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A Novel CD135+ Subset of Mouse Monocytes with a Distinct Differentiation Pathway and Antigen-Presenting Properties

Naoka Kamio1,2,3, Asumi Yokota3,4, Yuichi Tokuda5

  • 1Department of Clinical Laboratory Medicine, Kyoto University Hospital, Kyoto, Japan.

Journal of Immunology (Baltimore, Md. : 1950)
|July 15, 2022
PubMed

Insights

Researchers discovered a new type of antigen-presenting cell (APC) in mice: CD135+ monocytes. These cells are crucial components of the mononuclear phagocyte system (MPS), bridging innate and adaptive immunity.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The mononuclear phagocyte system (MPS), comprising monocytes/macrophages and dendritic cells (DCs), is vital for immune responses.
  • Recent findings suggest the MPS is more complex than previously understood, with novel cellular components emerging.

Purpose of the Study:

  • To identify and characterize novel cellular components within the mononuclear phagocyte system.
  • To investigate the role of CD135+ monocytes as potential antigen-presenting cells (APCs).

Main Methods:

  • Identification of CD135+ monocyte subset in mice.
  • Analysis of cell surface marker expression (MHC class II, CD209a).
  • Assessment of phagocytic activity and T lymphocyte activation capabilities.

Main Results:

  • A distinct CD135+ monocyte subset was identified in mouse bone marrow, spleen, and peripheral blood.
  • These monocytes expressed DC markers (MHC class II, CD209a) and monocyte/macrophage markers.
  • CD135+ monocytes demonstrated phagocytosis of bacteria and activation of naive T lymphocytes, confirming APC function.
  • These cells originated from macrophage DC progenitors, differentiating them from conventional monocytes.

Conclusions:

  • CD135+ monocytes represent a novel class of APCs within the MPS.
  • These findings expand our understanding of the cellular network regulating immune responses for host defense.

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