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A site-specific recombination function in Staphylococcus aureus plasmids.

M L Gennaro, J Kornblum, R P Novick

    Journal of Bacteriology
    |June 1, 1987
    PubMed
    Summary
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    Staphylococcal plasmids use a unique recombination site, RSA, for cointegrate formation. This process is recA-independent and mediated by the plasmid-encoded Pre protein, occurring specifically within the RSA core.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Staphylococcal plasmids commonly feature recombination sites facilitating site-specific cointegrate formation.
    • The RSA site, found on plasmids pT181 and pE194, comprises a 24-base pair core with flanking homologous regions of varying length.

    Purpose of the Study:

    • To investigate the mechanism of recombination at the RSA site.
    • To determine the host factors and plasmid-encoded proteins involved in RSA-mediated recombination.

    Main Methods:

    • Site-specific recombination assays were performed in recA-deficient bacterial hosts.
    • The role of the plasmid-encoded Pre (plasmid recombination) protein was assessed.
    • Recombination between intramolecular RSA sites was analyzed.

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    Main Results:

    • Recombination at the RSA site is independent of the host RecA protein.
    • A plasmid-encoded, trans-acting protein, Pre, mediates site-specific recombination at the RSA core.
    • Intramolecular recombination between two RSA sites was observed.
    • The Pre-RSA system does not appear to be involved in plasmid maintenance.

    Conclusions:

    • The Pre-mediated recombination system is a novel mechanism for site-specific DNA manipulation in staphylococcal plasmids.
    • This system functions independently of host recombination machinery, highlighting plasmid-specific genetic regulation.
    • The findings differentiate the Pre-RSA system from other plasmid-encoded recombination systems primarily involved in plasmid stability.