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miR-34a negatively regulates cell cycle factor Cdt2/DTL in HPV infected cervical cancer cells

Garima Singh1, Sonika Kumari Sharma1, Samarendra Kumar Singh2

  • 1Cell Cycle and Cancer Laboratory, School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, UP-221005, India.

BMC Cancer
|July 15, 2022
PubMed

Insights

MicroRNA miR-34a suppresses human papillomavirus E6 protein, destabilizing Cdt2 and inhibiting cervical cancer cell growth. This finding suggests miR-34a as a potential therapy for cervical cancer.

Area of Science:

  • Molecular Biology
  • Oncology
  • Virology

Background:

  • MicroRNAs regulate cellular processes, and their dysregulation is linked to cancer.
  • High-risk human papillomavirus (HR HPV) drives cervical cancer by targeting tumor suppressors and stabilizing oncogenic factors like Cdt2/DTL.
  • The precise mechanisms of microRNA-mediated regulation in HPV-infected cells are not fully understood.

Purpose of the Study:

  • To investigate the role of miR-34a in regulating cell cycle factors in HPV-infected cervical cancer cells.
  • To elucidate the mechanism by which miR-34a affects Cdt2/DTL protein levels and subsequent cellular events.

Main Methods:

  • Utilized HPV-infected cervical cancer cell lines.
  • Assessed the effect of miR-34a on HPV E6 protein levels.
  • Quantified Cdt2/DTL protein stability and levels of associated proteins (p21, Set8).
  • Evaluated the impact on cell proliferation, invasion, and migration.

Main Results:

  • miR-34a was found to suppress HPV E6 protein.
  • Suppression of E6 by miR-34a led to Cdt2/DTL destabilization.
  • Cdt2 destabilization resulted in increased p21 and Set8 levels, inhibiting cell proliferation, invasion, and migration.
  • Overexpression of HPV E6 or Cdt2 reversed the suppressive effects of miR-34a.

Conclusions:

  • miR-34a regulates the cell cycle factor Cdt2 by suppressing the viral HPV E6 protein.
  • This mechanism highlights a novel pathway for controlling HPV-driven cervical cancer.
  • miR-34a presents a promising therapeutic target for cervical cancer treatment.
Keywords:
Cdt2Cervical cancerHPV E6High-risk HPVmiR-34a

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