Surface molecularly imprinted solid-phase extraction for the determination of vancomycin in plasma samples using...

Area of Science:

• Analytical Chemistry
• Pharmacology
• Biomedical Science

Background:

• Vancomycin is a critical antibiotic for Gram-positive bacterial infections.
• Its narrow therapeutic index necessitates precise plasma concentration monitoring.
• Existing analytical methods may lack the required sensitivity or reliability.

Purpose of the Study:

• To develop and validate a sensitive and reliable analytical method for vancomycin determination in plasma.
• To establish a robust method for therapeutic drug monitoring of vancomycin.
• To facilitate accurate pharmacokinetic studies of vancomycin.

Main Methods:

• Surface molecularly imprinted solid-phase extraction (SMIP-SPE) for sample cleanup.
• Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for sensitive detection.
• Validation of the method using plasma samples from mice.

Main Results:

• The method demonstrated excellent linearity (r=0.9993) over a calibration range of 1-100 ng/mL.
• Average recoveries ranged from 94.3% to 104.0%, with precision below 10.5%.
• The limit of detection (LOD) was 0.5 ng/mL and the limit of quantification (LOQ) was 1 ng/mL.

Conclusions:

• The developed SMIP-SPE combined with LC-MS/MS is a sensitive, reliable, and efficient method for vancomycin quantification in plasma.
• This validated method is suitable for therapeutic drug monitoring and pharmacokinetic studies.
• The method was successfully applied to detect vancomycin in mice following oral administration.