Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

5.1K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.1K
Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

2.0K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacterial Pathogen Identification and Its Association with Clinical, Ultrasonographic, and Post-Mortem Severity in Lacaune Lambs with Ovine Respiratory Complex.

Animals : an open access journal from MDPI·2026
Same author

XLA-MTS: a distinct clinical genetic entity characterized by immunodeficiency and neurodevelopmental delay.

Orphanet journal of rare diseases·2026
Same author

Epigenetic constraints and enhancer innovation link neuronal plasticity to evolutionary adaptation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Bioaccessibility of phloretin and its derivatives using a dynamic in vitro digester. Effects on glucose uptake, bacterial growth and anti-inflammatory activity.

Food research international (Ottawa, Ont.)·2026
Same author

The aging factor EPS8 induces disease-related protein aggregation through RAC signaling hyperactivation.

Nature aging·2025
Same author

A postnatal molecular switch drives activity-dependent maturation of parvalbumin interneurons.

Cell·2025

Related Experiment Video

Updated: Sep 4, 2025

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
07:51

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Published on: April 30, 2014

20.5K

MOTS-c promotes muscle differentiation in vitro.

Sandra García-Benlloch1, Francisco Revert-Ros2, Jose Rafael Blesa2

  • 1Facultad de Medicina y Odontología, Universidad Católica de Valencia San Vicente Mártir, c/Quevedo 2, 46001 Valencia, Spain; Escuela de Doctorado, Universidad Católica de Valencia San Vicente Mártir, c/ Quevedo 2, 46001 Valencia, Spain.

Peptides
|July 16, 2022
PubMed
Summary

Mitochondrial peptide MOTS-c enhances muscle progenitor cell differentiation and myotube formation by interacting with the STAT3 pathway. This peptide shows potential for treating muscle-wasting diseases.

Keywords:
AtrophyMitochondria derived peptidesMuscle

More Related Videos

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
06:53

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

Published on: October 15, 2019

17.6K
Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

9.9K

Related Experiment Videos

Last Updated: Sep 4, 2025

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
07:51

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Published on: April 30, 2014

20.5K
Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
06:53

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

Published on: October 15, 2019

17.6K
Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

9.9K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Physiology

Background:

  • MOTS-c (mitochondrial open reading frame of the 12 S rRNA-c) is a peptide hormone with known roles in metabolic homeostasis.
  • Its specific effects on muscle tissue and the underlying mechanisms remain largely unexplored.

Purpose of the Study:

  • To investigate the role of MOTS-c in muscle progenitor cell differentiation.
  • To elucidate the molecular mechanism by which MOTS-c influences myogenesis.

Main Methods:

  • Human (LHCN-M2) and murine (C2C12) muscle progenitor cells were treated with wild-type MOTS-c and a Y8F mutant peptide.
  • Myotube formation, myogenin staining, and STAT3 transcriptional activity were assessed.
  • In silico analysis identified a putative SH2 binding motif in MOTS-c.

Main Results:

  • Wild-type MOTS-c significantly increased myotube formation in both cell models.
  • MOTS-c protected against IL-6-induced reduction of myogenin staining.
  • MOTS-c, but not the Y8F mutant, blocked IL-6-induced STAT3 transcriptional activity.

Conclusions:

  • MOTS-c enhances muscle progenitor cell differentiation and myotube formation.
  • This effect is mediated by the interaction of MOTS-c with STAT3 via its YIFY region, inhibiting STAT3 transcriptional activity.
  • MOTS-c represents a potential therapeutic target for muscle-wasting conditions.