Uptake and metabolism of ¹⁴C-triclosan in celery under hydroponic system

As triclosan is used extensively as an antimicrobial agent, it inevitably enters agroecosystems, when sewage and treated wastewater are applied to agricultural fields. As a result, triclosan can be accumulated into crops and vegetables. Currently, limited information is available on the metabolism of triclosan in vegetables. In this study, the fate of ¹⁴C-triclosan in celery under a hydroponic system was investigated in a 30-day laboratory test. Most (97.7 %) of the ¹⁴C-triclosan accumulated in celery. The bioconcentration factors of triclosan were up to 3140 L kg^-1 at day 30. The concentration of ¹⁴C-triclosan in roots (17.8 mg kg^-1) was 57- and 127-fold higher than that in stems (0.31 mg kg^-1) and leaves (0.14 mg kg^-1), respectively, at day 30, suggesting a higher accumulation of triclosan in celery roots and negligible transport to stems and leaves. Moreover, triclosan, as well as its eight metabolites, was detected and identified in celery tissues and the growth medium using ¹⁴C-labelling and LC-Q-TOF-MS analysis methods. Phase I metabolites in the growth medium were from hydroxylation, dechlorination, nitration, and nitrosylation. Phase II metabolism was the major pathway in celery tissues. Monosaccharide, disaccharide, and sulfate conjugates of triclosan were putatively identified. The results represent an important step toward a better evaluation of the behavior of triclosan in vegetables, with notable implications for environmental and human risk assessments of triclosan.