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Updated: Sep 4, 2025

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
Published on: October 20, 2019
Factors influencing mosaicism: a retrospective analysis
Qiu-Xiang Huang1, Zhi-Hong Wang2, Wu-Jian Huang1
1Center of Reproductive Medicine, Department of Obstetrics and Gynecology, 900th Hospital of the Joint Logistics Team, Fuzhou Fujian, People's Republic of China.
Research Question:
What factors affect the incidence of mosaic embryos resulting from assisted reproductive technology?
Design:
A retrospective analysis of data from preimplantation genetic testing for aneuploidies in 544 couples was conducted using data from an electronic medical record database.
Results:
Of 1910 embryos studied, 127 (6.6%) were mosaic. In multivariable logistic regression analysis, mosaicism incidence increased in embryos from IVF versus intracytoplasmic sperm injection (ICSI) (odds ratio [OR] 4.560, 95% confidence interval [CI] 2.800-7.424, P < 0.001), and in embryos from abnormal versus normal semen (OR 3.496, 95% CI 2.455-4.979, P < 0.001). Embryos tested using SurePlex 24Sure had lower mosaicism percentages than those tested using MALBAC-NGS and PicoPLEX GenetiSure (OR 2.726, 95% CI 1.532-4.852, P = 0.001; OR 2.389, 95% CI 1.537-3.711, P < 0.001, respectively).
Conclusions:
Semen quality, fertilization method and detection system are independent factors associated with embryonic mosaicism.
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