Ibuprofen Alleviates Acute Pancreatitis- (AP-) Induced Myocardial Injury by Inhibiting AIM2

Area of Science:

• Biochemistry
• Pathology
• Pharmacology

Background:

• Acute pancreatitis (AP) can lead to myocardial injury due to metabolic disturbances.
• Trace element deficiencies may exacerbate myocardial damage in AP.
• The precise mechanisms underlying AP-induced myocardial injury require further elucidation.

Purpose of the Study:

• To investigate the protective mechanism of ibuprofen against myocardial injury in AP.
• To explore the role of AIM2 in AP-induced myocardial inflammation and injury.

Main Methods:

• Established AP cell and rat models.
• Utilized HE staining, ELISA, qRT-PCR, and Western blot to assess histological changes, cytokine levels, and gene/protein expression.
• Quantified levels of TNF-α, IL-6, LDH, and CK-MB.

Main Results:

• AIM2 expression was significantly upregulated in AP cells.
• Downregulating AIM2 reduced inflammation and myocardial injury in AP models.
• Ibuprofen inhibited AIM2 expression, thereby alleviating inflammation and myocardial injury in both cell and rat models.

Conclusions:

• Ibuprofen mitigates myocardial injury in AP by suppressing AIM2 expression.
• Targeting AIM2 presents a potential therapeutic avenue for managing AP-related cardiac complications.