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Purification of human interferons by antibody affinity chromatography

K Berg, R Alacam, R D Hamilton

    Texas Reports on Biology and Medicine
    |January 1, 1977
    PubMed

    Human Leukocyte Interferon, Human Fibroblast Interferon and Namalva-interferon was purified on an antibody affinity colomn up to 2-20 X 10 IFU/mg protein with recoveries in the 80-120% range. The colomn was made by coupling highly absorped anti-leukocyte interferon immunoglobulins to Sepharose 4B. The absorption was performed by means of crude human leukocyte interferon covalently bound to Sepharose 4B.

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