Purification of human interferons by antibody affinity chromatography

Area of Science:

• Immunology
• Biochemistry
• Protein Purification

Background:

• Interferons are critical antiviral proteins with therapeutic potential.
• Efficient purification methods are essential for obtaining high-quality interferons for research and clinical applications.

Purpose of the Study:

• To develop and validate an antibody affinity chromatography method for purifying human interferons.
• To assess the purity and recovery of purified interferons using this novel technique.

Main Methods:

• Antibody affinity column preparation by coupling anti-leukocyte interferon immunoglobulins to Sepharose 4B.
• Purification of Human Leukocyte Interferon, Human Fibroblast Interferon, and Namalva-interferon using the prepared antibody affinity column.
• Quantification of interferon purity and recovery rates.

Main Results:

• Achieved high purity levels for Human Leukocyte Interferon, Human Fibroblast Interferon, and Namalva-interferon, ranging from 2-20 X 10 IFU/mg protein.
• Demonstrated high recovery rates for the purified interferons, within the 80-120% range.
• Successfully utilized antibody affinity chromatography for effective interferon purification.

Conclusions:

• Antibody affinity chromatography is a highly effective method for purifying human interferons.
• The developed method provides high purity and excellent recovery, suitable for producing interferons for therapeutic and research purposes.