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Long Noncoding RNA LINC01554 Inhibits the Progression of NSCLC Progression by Functioning as a ceRNA for miR-1267 and

Zizong Wang1, Bin Yang2, Jin Zhang3

  • 1Department of Thoracic Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.

Biomed Research International
|July 18, 2022
PubMed
Abstract

Insights

LINC01554, a long non-coding RNA, suppresses non-small cell lung cancer (NSCLC) growth and metastasis by targeting miR-1267 and the ING3/Akt/mTOR pathway. This finding highlights LINC01554 as a potential therapeutic target for NSCLC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality worldwide.
  • Understanding the molecular mechanisms driving NSCLC progression is crucial for developing effective therapeutic strategies.

Purpose of the Study:

  • To investigate the biological functions and mechanisms of action of the long non-coding RNA LINC01554 in non-small cell lung cancer (NSCLC).
  • To evaluate the prognostic value of LINC01554 in NSCLC patients.

Main Methods:

  • Analysis of LINC01554 expression and prognostic value in NSCLC using The Cancer Genome Atlas (TCGA) datasets.
  • In vitro and in vivo assays (MTT, colony formation, wound healing, transwell, in vivo assays) to assess the role of LINC01554 in NSCLC.
  • Dual-luciferase reporter assays to elucidate the regulatory relationship between LINC01554, miR-1267, and ING3, and western blotting to measure protein expression.

Main Results:

  • LINC01554 expression was found to be downregulated in NSCLC and correlated with poorer prognosis.
  • Overexpression of LINC01554 inhibited NSCLC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT).
  • LINC01554 directly targets miR-1267, which in turn targets ING3. This axis regulates the Akt/mTOR signaling pathway, suppressing tumor growth and metastasis.

Conclusions:

  • This study provides the first evidence for the involvement of the LINC01554/miR-1267/ING3 axis in regulating NSCLC proliferation and metastasis via the Akt/mTOR pathway.
  • LINC01554 demonstrates potential as a novel therapeutic target for NSCLC treatment.

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