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Related Concept Videos

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Myosins are a family of molecular motor proteins, first identified in the skeletal muscles, where they are responsible for muscle contraction. Along with their role in muscle contraction, these proteins also play a role in the intracellular transport of molecules and vesicles. There are twenty-four classes of myosins based on their domain sequence and organization. Of the twenty-four, six classes (Myosin I, Myosin II, Myosin V, Myosin VI, Myosin VII, and Myosin X)  have been well...
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The nervous system consists of complex motor neuron circuits, including upper motor neurons originating from the cerebral cortex and lower motor neurons starting in the spinal cord, coordinating both voluntary and involuntary movements. Among these, somatic motor neurons activate skeletal muscles and are classified into alpha, beta, and gamma types. Alpha neurons are vital for voluntary movement coordination, while gamma neurons adjust muscle spindle sensitivity, and the function of beta...
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Related Experiment Video

Updated: Sep 4, 2025

Bladder Smooth Muscle Strip Contractility as a Method to Evaluate Lower Urinary Tract Pharmacology
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Myosin 5a in the Urinary Bladder: Localization, Splice Variant Expression, and Functional Role in Neurotransmission.

Josephine A Carew1,2,3, Vivian Cristofaro1,2,3, Suhas P Dasari1

  • 1Urology Research, VA Boston Healthcare System, Boston, MA, United States.

Frontiers in Physiology
|July 18, 2022
PubMed
Summary

Myosin 5a facilitates neurotransmission in the bladder by transporting synaptic vesicles. Altered Myosin 5a splicing in DBA mice impairs bladder nerve function and contractile responses.

Keywords:
Myosin 5abladder smooth musclemyosin motorneurotransmissionperipheral nerveprotein splice variants

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Area of Science:

  • Neuroscience
  • Urology
  • Cell Biology

Background:

  • Neurotransmission dysregulation is implicated in lower urinary tract conditions.
  • Mechanisms of neurotransmitter release in the bladder are not fully understood.
  • Myosin 5a is a motor protein involved in synaptic vesicle transport.

Purpose of the Study:

  • To investigate the role of Myosin 5a in bladder neurotransmission.
  • To examine Myosin 5a expression and function in mouse bladders.

Main Methods:

  • Quantitative PCR and restriction analyses to assess Myosin 5a expression in DBA and control mouse bladders.
  • Ex vivo isometric tension analysis to evaluate contractile responses to nerve stimulation.

Main Results:

  • Myosin 5a was localized in cholinergic nerve fibers in the bladder.
  • DBA mouse bladders showed altered Myosin 5a splice variant expression and reduced full-length transcripts.
  • DBA bladders exhibited attenuated neurally-mediated contractile responses compared to controls.

Conclusions:

  • Myosin 5a plays a crucial role in facilitating excitatory neurotransmission in the urinary bladder.
  • The expression and abundance of Myosin 5a splice variants are critical for efficient synaptic vesicle transport and bladder function.