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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
Published on: September 6, 2014
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Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency.
Guanxu Ji1, Xiaoxiao Xiao1, Min Huang1
1The State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China.
Heliyon
|July 18, 2022
Summary
Jmjd6 protein is crucial for maintaining embryonic stem cell identity and function. Its depletion impairs pluripotency and somatic cell reprogramming, highlighting its regulatory role in cell homeostasis.
Area of Science:
- Molecular Biology
- Stem Cell Biology
- Epigenetics
Background:
- Jmjd6 (Jumonji domain-containing 6) is a nuclear protein with known histone arginine demethylation and lysyl hydroxylase activities.
- Jmjd6 is essential for cell differentiation and embryonic development.
- The specific role of Jmjd6 in mammalian embryonic stem cell (ES cell) identity and cellular reprogramming remains largely uncharacterized.
Purpose of the Study:
- To investigate the function of Jmjd6 in maintaining mammalian ES cell identity.
- To determine the impact of Jmjd6 on somatic cell reprogramming efficiency.
Main Methods:
- Depletion of Jmjd6 using specific techniques.
- Analysis of pluripotency gene expression.
- Assessment of apoptosis, glycolysis, and cell cycle progression.
- Measurement of BrdU incorporation.
- Evaluation of reprogramming efficiency in mouse embryonic fibroblasts (MEFs) with altered Jmjd6 levels.
Main Results:
- Jmjd6 depletion led to downregulation of pluripotency genes in ES cells.
- Jmjd6 deficiency was associated with increased apoptosis, altered glycolysis, and cell cycle changes.
- Reduced bromodeoxyuridine (BrdU) incorporation was observed in Jmjd6-depleted cells, indicating effects on DNA synthesis.
- Overexpression of Jmjd6 enhanced the efficiency of somatic cell reprogramming, while its depletion reduced efficiency.
Conclusions:
- Jmjd6 plays a significant role in regulating ES cell homeostasis.
- Jmjd6 is a key factor that can enhance somatic cell reprogramming.
- These findings establish Jmjd6 as a critical regulator of stem cell identity and reprogramming potential.
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