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A Sperm Quality Detection System Based on Microfluidic Chip and Micro-Imaging System.

Xiaoqing Pan¹, Kang Gao², Ning Yang²

¹Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

Frontiers in Veterinary Science

|July 18, 2022

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A new portable system using microfluidic chips and OpenCV algorithms can assess livestock sperm quality, including motility and survival rates. This technology offers a fast, accurate, and portable solution for reproductive applications.

Area of Science:

Veterinary Science
Biomedical Engineering
Microfluidics

Background:

Sperm quality assessment is crucial for livestock reproductive efficiency.
Current methods for sperm analysis lack portability and real-time capabilities.
Accurate sperm evaluation is vital for artificial insemination and in vitro fertilization success.

Purpose of the Study:

To develop a portable microscopic imaging system for comprehensive livestock sperm quality evaluation.
To enhance the real-time and portability of sperm quality detection.
To assess sperm vitality and survival rates using a microfluidic chip-based system.

Main Methods:

Designed a microfluidic chip for automated sample mixing.
Developed a 400x magnification optical system for microscopic sperm observation.

Keywords:

micro-imaging system microfluidic chip motility sperm quality survival rate

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Implemented OpenCV-based algorithms for identifying sperm motility and survival rates.

Main Results:

The system achieved 94.0% accuracy in detecting sperm survival rate with a 0.6% error rate.
Sperm motility evaluation results were consistent with traditional computer-aided sperm analysis (CASA).
The system demonstrated a rapid identification time of 9 seconds.

Conclusions:

The developed portable system provides a comprehensive and efficient method for livestock sperm quality assessment.
The system's accuracy, speed, and portability make it highly suitable for field applications in animal reproduction.
This technology has significant potential to improve artificial insemination and in vitro fertilization programs.