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An Altered Metabolism in Leukocytes Showing in vitro igG Memory From SARS-CoV-2-Infected Patients

G Fanelli1, F Gevi1, G Zarletti2

  • 1Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy.

Frontiers in Molecular Biosciences
|July 18, 2022
PubMed

Insights

COVID-19 infection impacts cell metabolism. Researchers found elevated S-adenosyl-Homocysteine, Sarcosine, and Arginine in leukocytes with SARS-CoV-2 IgG memory, suggesting a new measure for understanding viral effects.

Area of Science:

  • Immunology
  • Metabolomics
  • Virology

Background:

  • Coronavirus disease 2019 (COVID-19) is a systemic viral infection with known metabolic consequences.
  • Understanding the long-term effects of SARS-CoV-2 on immune cells is crucial for managing post-infection sequelae.

Purpose of the Study:

  • To investigate the metabolic profiles of peripheral blood mononuclear cells (PBMCs) in individuals with and without SARS-CoV-2 IgG memory.
  • To identify specific metabolites associated with immune memory following COVID-19 infection.

Main Methods:

  • Metabolomic profiling using mass spectrometry was performed on 41 *in vitro* PBMC cultures.
  • Samples were analyzed from individuals 60-90 days post-infection, with 17 showing IgG memory for the spike-S1 antigen.

Main Results:

  • A significant upregulation of S-adenosyl-Homocysteine, Sarcosine, and Arginine was observed in leukocytes with IgG memory.
  • These identified metabolites are implicated in viral recovery and immune responses.

Conclusions:

  • The study identified key metabolites (S-adenosyl-Homocysteine, Sarcosine, Arginine) associated with SARS-CoV-2 immune memory.
  • These findings may offer a novel, accessible method for assessing the impact of SARS-CoV-2 on leukocyte metabolism and immune function.

Keywords:
COVID-19cell-ELISAin vitro B-cell memorymass spectrometrymetabolomics

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