miR-181a-5p can inhibit the proliferation and promote the differentiation of chicken primary myoblasts

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Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
07:51

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Published on: April 30, 2014

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P Yuan1, S Fan1, B Zhai1

  • 1College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan, China.

British Poultry Science
|July 18, 2022
PubMed

Insights

MicroRNAs (miRNAs) regulate skeletal muscle development. This study shows miR-181a-5p inhibits chicken myoblast proliferation and promotes differentiation, impacting muscle growth and meat quality.

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Animal Science

Background:

  • Myoblast proliferation and differentiation are critical for skeletal muscle development.
  • MicroRNAs (miRNAs) are key regulators of these cellular processes.
  • Understanding miRNA roles is essential for improving meat quality traits.

Purpose of the Study:

  • To investigate the role of miR-181a-5p in chicken primary myoblast (CPM) development.
  • To determine the effect of miR-181a-5p on myoblast proliferation and differentiation.
  • To identify potential target genes of miR-181a-5p involved in muscle development.

Main Methods:

  • Detection of miR-181a-5p expression in Gushi chickens across different tissues, ages, and differentiation stages.
  • Cell proliferation assays (CCK-8, EdU) and cell cycle analysis on CPMs.
  • Analysis of differentiation markers (MYOD, MYOG, MYHC) and myotube formation via immunofluorescence.
  • mRNA transcriptome analysis to identify potential miR-181a-5p target genes.

Main Results:

  • miR-181a-5p exhibited differential expression in Gushi chickens.
  • miR-181a-5p significantly inhibited CPM proliferation and promoted differentiation, indicated by increased MYOD, MYOG, and MYHC expression and larger myotube areas.
  • 63 potential target genes were identified, with ASNS, SMYD1, and FOS implicated in muscle development regulation.

Conclusions:

  • miR-181a-5p acts as a negative regulator of chicken myoblast proliferation and a positive regulator of differentiation.
  • This miRNA influences skeletal muscle development potentially through target genes like ASNS, SMYD1, and FOS.
  • The findings provide a foundation for understanding miR-181a-5p's role in Gushi chicken muscle development and meat quality.
Keywords:
Gushi chickendifferentiationmiR-181a-5pmyoblastproliferation

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