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Related Experiment Videos

Piccolyte investments for better section cutting.

G F Hammond, B Beckman

    Stain Technology
    |March 1, 1978
    PubMed
    Summary

    Piccolyte 115, a beta-pinene polymer, improves tissue embedding. It enhances section quality and reduces processing time when used with standard embedding media like paraffin.

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    Area of Science:

    • Histology and Tissue Processing
    • Polymer Science in Biological Applications

    Background:

    • Traditional paraffin embedding can be time-consuming and may result in tissue sections with folds or wrinkles.
    • The development of improved embedding media is crucial for efficient and high-quality histological analysis.

    Purpose of the Study:

    • To evaluate the efficacy of Piccolyte 115 as an additive to standard tissue embedding media.
    • To determine if Piccolyte 115 can enhance the quality and speed of tissue sectioning.

    Main Methods:

    • Piccolyte 115 (beta-pinene polymers) was added to Tissuemat, Paraplast, and Peel-Away embedding media.
    • It was also mixed with paraffin at 3% and 10% concentrations for double embedding of paraffin-infiltrated tissues.

    Main Results:

    • Piccolyte 115 facilitates the investment of infiltrated tissues.
    • When used in double embedding with paraffin (3% and 10%), it produced good, complete tissue sections.
    • Sections were virtually free of folds or wrinkles, achieved in less time and with less effort compared to paraffin alone.

    Conclusions:

    • Piccolyte 115 is a valuable additive for tissue embedding media.
    • Its use can significantly improve the quality of tissue sections and streamline the histological processing workflow.

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