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Updated: Sep 4, 2025

Cell-Free Dot Blot as a Practical and Adaptable Immunoassay Platform for the Detection of Antibody Response in Human and Animal Sera
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Cell-Based Platform for Antigen Testing and Its Application for SARS-CoV-2 Infection.

Marvin A Ssemadaali1, Sherri Newmyer1, Harikrishnan Radhakrishnan1

  • 1Biosciences Division, SRI Internationalgrid.98913.3a, Menlo Park, California, USA.

Microbiology Spectrum
|July 19, 2022
PubMed
Summary
This summary is machine-generated.

We developed DxCell, a novel cell-based antigen test for rapid diagnosis of SARS-CoV-2 infections. This self-replicative platform offers label-free, sample-free testing for decentralized healthcare facilities, aiding pandemic mitigation.

Keywords:
CAR T-cellCOVID-19 diagnosticsantigen testcell-based sensorsreporter cell

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Area of Science:

  • Biotechnology
  • Virology
  • Diagnostics

Background:

  • Pandemic mitigation relies on rapid, accessible testing infrastructure.
  • Current testing methods often lack the speed and decentralization needed for effective pandemic response.
  • Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infections require innovative diagnostic solutions.

Purpose of the Study:

  • To engineer a cell-based antigen test platform (DxCell) for rapid diagnosis of SARS-CoV-2.
  • To enable deployment in decentralized healthcare settings for continuous testing.
  • To provide a label-free, sample-processing-free diagnostic tool.

Main Methods:

  • Engineered a fast-growing cell line into a DxCell platform presenting SARS-CoV-2 spike glycoprotein.
  • Validated the DxCell using SARS-CoV-2-infected cells and oropharyngeal swabs from infected mice.
  • Benchmarked DxCell performance against quantitative PCR (qPCR) for accuracy.

Main Results:

  • DxCell quantitatively detected SARS-CoV-2 antigens within 1 hour with high sensitivity (P < 0.02).
  • Reporter signal intensity correlated with the number of SARS-CoV-2 spike glycoprotein cells, indicating infection burden.
  • DxCell accurately differentiated between infected and control samples, comparable to qPCR (P < 0.05).

Conclusions:

  • Developed a novel, self-replicative cell-based antigen test platform (DxCell) for SARS-CoV-2.
  • DxCell offers advantages like label-free detection and no sample processing, suitable for decentralized healthcare.
  • The platform demonstrates broad applicability for detecting various viral antigens, enhancing pandemic preparedness.