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Visualizing Bacteria in Nematodes using Fluorescent Microscopy
Published on: October 19, 2012
Characterization of a Root-Knot Nematode Infecting Aconitum carmichaelii in Southwest China
Wentao Wu1, Kunhao Ye1,2, Shaofang Zhou1
1College of Plant Protection, Yunnan Agricultural University, Key Laboratory of Agricultural Biodiversity and Pest Control, Yunnan Kunming 650201, China.
Abstract:
Growth of the Chinese herbal medicine industry has resulted in several new pests and diseases. China is one of the world largest producers of monkshood (Aconitum carmichaelii Debx.), but an unidentified root-knot nematode has become a significant pest in the southwestern provinces of Yunnan and Sichuan. Morphological characteristics and the ribosomal DNA-internal transcribed spacer and D2-D3 region of the 28S ribosomal RNA gene sequences were used to identify the nematode as Meloidogyne hapla. Through investigation, this is the first report of M. hapla infecting monkshood in Yunnan and Sichuan Provinces.

