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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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The splicing factor SF3B4 drives proliferation and invasion in cervical cancer by regulating SPAG5

Yingwei Li1,2, Yuchao Diao3,4, Zixiang Wang3

  • 1Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China. sduliyingwei@126.com.

Cell Death Discovery
|July 19, 2022
PubMed

Insights

Splicing factor 3b subunit 4 (SF3B4) promotes cervical cancer (CC) progression by affecting sperm-associated antigen 5 (SPAG5) splicing. SF3B4 may be a potential therapeutic target for cervical cancer treatment.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Alternative splicing (AS) regulation by the splicing factor 3b (SF3B) family is crucial in cancer.
  • The specific role of SF3B family members in cervical cancer (CC) requires further investigation.

Purpose of the Study:

  • To elucidate the biological function of SF3B4 in cervical cancer.
  • To identify downstream targets and mechanisms of SF3B4 in CC progression.

Main Methods:

  • Bioinformatics analysis of The Cancer Genome Atlas (TCGA) CESC data.
  • In vitro and in vivo experiments assessing cell proliferation and invasion.
  • RNA sequencing (RNA-seq) and alternative splicing analysis.

Main Results:

  • SF3B4 was highly expressed in CC and promoted CC cell proliferation and invasion.
  • SF3B4 regulated the effective splicing of its downstream target, sperm-associated antigen 5 (SPAG5), leading to reduced SPAG5 maturation.
  • SPAG5 deficiency counteracted the oncogenic effects of SF3B4 overexpression in CC cells.

Conclusions:

  • SF3B4 promotes cervical cancer progression through the regulation of SPAG5 splicing.
  • SF3B4 represents a potential therapeutic target for cervical cancer.

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