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Jujuboside B post-treatment attenuates PM2.5-induced lung injury in mice
Soo Ho Ryu1, Nayeon Kim1, Chaeyeong Kim1
1College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, Republic of Korea.
Abstract:
Fine particulate matter (PM2.5) is an air pollutant that causes severe lung injury. We investigated the effects of Jujuboside B (JB), a component of Zizyphi Spinosi Semen, on lung toxicity caused by PM2.5, and we identified the mechanism of its protective effect. Lung injury in an animal model was induced by intratracheal administration of a PM2.5 suspension. After 2 days of PM2.5 pretreatment, mice were administered JB via the tail vein three times over a 2-day period. JB significantly reduced the histological lung damage as well as the lung wet/dry weight ratio. JB also considerably reduced PM2.5-induced autophagy dysfunction, apoptosis, inflammatory cytokine levels, and the number of PM2.5-induced lymphocytes in the bronchial alveolar fluid. We conclude that by regulating TLR2, 4-MyD88, and mTOR-autophagy pathways, JB exerts a protective effect on lung injury. Thus, JB can be used as a potential therapeutic agent for PM2.5-induced lung damage.
Insights
Jujuboside B (JB) protects against lung injury caused by fine particulate matter (PM2.5). This study shows JB reduces inflammation and cell damage, suggesting it as a potential therapy for PM2.5-induced lung damage.
Area of Science:
- Environmental Health
- Toxicology
- Pharmacology
Background:
- Fine particulate matter (PM2.5) is a major air pollutant linked to severe lung injury.
- PM2.5 exposure can lead to inflammation, autophagy dysfunction, and apoptosis in lung tissues.
- Zizyphi Spinosi Semen contains Jujuboside B (JB), a compound with potential therapeutic properties.
Purpose of the Study:
- To investigate the protective effects of Jujuboside B (JB) against PM2.5-induced lung toxicity.
- To elucidate the underlying mechanisms of JB's protective action in a mouse model.
Main Methods:
- An animal model was established using intratracheal administration of PM2.5 suspension to induce lung injury.
- Mice received PM2.5 followed by tail vein injections of JB over a 2-day period.
- Histological analysis, lung wet/dry weight ratio, and assessment of autophagy, apoptosis, inflammatory cytokines, and lymphocytes were performed.
Main Results:
- JB significantly reduced histological lung damage and the lung wet/dry weight ratio.
- JB ameliorated PM2.5-induced autophagy dysfunction, apoptosis, and elevated inflammatory cytokine levels.
- JB decreased the number of lymphocytes in bronchial alveolar fluid caused by PM2.5 exposure.
Conclusions:
- Jujuboside B (JB) demonstrates significant protective effects against PM2.5-induced lung injury.
- JB exerts its protective effects by modulating TLR2, 4-MyD88, and mTOR-autophagy pathways.
- JB is a potential therapeutic agent for treating lung damage caused by PM2.5 air pollution.
