Related Experiment Video
Updated: Sep 4, 2025
13:21
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
Published on: June 16, 2017
10.1K
Abnormal GRHL2 Methylation Confers Malignant Progression to Acute Leukemia
Jing Hua1, Congcong Ma2, Chao Hui Wang3
1Department of Hematology, Shandong Provincial Qianfoshan Hospital, Shandong University, China.
Applied Bionics and Biomechanics
|July 20, 2022
Summary
Grainyhead-like 2 (GRHL2) is downregulated and hypermethylated in acute leukemia, correlating with poor prognosis. Demethylating agents like arsenic trioxide and 5-azacitidine inhibit leukemia cell proliferation and affect the TGF-β pathway.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Abnormal methylation of Grainyhead-like 2 (GRHL2) is linked to cancer malignancy.
- Understanding GRHL2's role in acute leukemia is crucial for prognosis.
Purpose of the Study:
- To investigate the abnormal expression and methylation of GRHL2 in acute leukemia.
- To explore the relationship between GRHL2 and patient prognosis.
- To assess the therapeutic potential of demethylating agents on GRHL2 expression and leukemia cell behavior.
Main Methods:
- Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting for GRHL2 expression.
- Methylation-specific polymerase chain reaction (MSP) for GRHL2 promoter methylation.
- Treatment of leukemia cell lines with arsenic trioxide and 5-azacitidine.
- Analysis of clinicopathological features and patient survival data.
Main Results:
- GRHL2 expression was significantly lower in acute leukemia patients and cell lines compared to controls.
- Lower GRHL2 expression correlated with adverse clinicopathological features and poorer overall survival.
- GRHL2 promoter methylation was significantly higher in acute leukemia.
- Demethylating agents increased GRHL2 expression and inhibited leukemia cell proliferation, impacting the TGF-β pathway.
Conclusions:
- GRHL2 dysregulation (low expression, high methylation) is associated with poor prognosis in acute leukemia.
- GRHL2 plays a significant role in acute leukemia development and progression.
- Targeting GRHL2 methylation may offer a therapeutic strategy for acute leukemia.
Related Concept Videos
01:46Epigenetic Regulation
31.3K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.3K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
02:07Tumor Progression
6.5K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.5K