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Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area
Komal Chillar1, Yipeng Yin1, Adikari M Dhananjani N Eriyagama1
1Department of Chemistry, Michigan Technological University, Houghton, MI, United States.
Abstract:
Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD260). Here, we describe a method for direct determination of OD260 of ODNs using the area of the peaks in HPLC profiles. It is expected that the method will save significant time for researchers in the area of nucleic acid research, and minimize the loss of oligonucleotide samples.
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