Related Experiment Video

Updated: Sep 4, 2025

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
11:02

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Published on: October 30, 2013

21.4K

TBX3 stimulates proliferation and stem cell self-renewal in bladder carcinoma

Lifu Huang1,2, Wenfei Shao2,3, Xiaohong Wang2,4

  • 1Department of Urology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai City, Zhejiang Province, China.

Histology and Histopathology
|July 20, 2022
PubMed
Abstract

Insights

TBX3, a gene influenced by TGF-β1, fuels bladder carcinoma growth and stemness while hindering apoptosis. Targeting TBX3 offers a promising strategy for bladder cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Stem Cell Research

Background:

  • Bladder carcinoma is a leading cause of cancer death in men, with high rates of recurrence and metastasis after surgery.
  • Current treatments for bladder carcinoma have limitations, necessitating the identification of novel therapeutic targets.

Purpose of the Study:

  • To investigate the role of TBX3 in bladder carcinoma.
  • To explore the potential of TBX3 as a therapeutic target for bladder cancer.

Main Methods:

  • Assessed TBX3 protein and mRNA expression in bladder carcinoma tissues and cells via Western blot and qRT-PCR.
  • Isolated cancer stem cells (CSCs) and analyzed stemness-associated proteins (CD44, CD24, ESA).
  • Evaluated cell proliferation, apoptosis, and self-renewal capabilities using CCK-8, colony formation, and sphere formation assays.

Main Results:

  • Elevated TBX3 expression correlated with increased bladder carcinoma cell proliferation and decreased apoptosis.
  • TBX3 and stemness markers were highly expressed in bladder cancer stem cells.
  • TBX3 promoted CSC self-renewal and inhibited apoptosis, with TGF-β1 identified as a regulator of TBX3.

Conclusions:

  • TBX3, regulated by TGF-β1, drives bladder carcinoma cell proliferation, inhibits apoptosis, and enhances stemness.
  • TBX3 represents a potential therapeutic target for bladder cancer, particularly targeting cancer stem cells.

More Related Videos

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer09:24

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

9.1K
An Orthotopic Bladder Cancer Model for Gene Delivery Studies07:48

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

Published on: December 1, 2013

12.6K

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.7K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.3K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
5.0K