A Ti₃ C₂ T_X /Pt-Pd based amperometric biosensor for sensitive cancer biomarker detection

Area of Science:

• Nanomaterials Science
• Biomedical Engineering
• Analytical Chemistry

Background:

• Early cancer detection relies on identifying specific biomarkers.
• Sarcosine in blood/urine is a potential indicator for prostate cancer diagnosis.
• Existing sarcosine detection methods lack simplicity, precision, and wide applicability.

Purpose of the Study:

• To develop a highly stable and sensitive sarcosine biosensor.
• To utilize a novel Ti3C2Tx/Pt-Pd nanocomposite for enhanced electrochemical performance.
• To establish a reliable method for prostate cancer biomarker detection.

Main Methods:

• Synthesis of Ti3C2Tx/Pt-Pd nanocomposite via one-step alcohol reduction.
• Fabrication of a sarcosine biosensor (GCE/Ti3C2Tx/Pt-Pd/SOx) on a glassy carbon electrode.
• Electrochemical characterization and performance evaluation of the biosensor.

Main Results:

• The biosensor demonstrated excellent stability and electrochemical activity.
• A wide linear detection range for sarcosine (1-1000 µM) was achieved.
• Low limit of detection (0.16 µM) and high sensitivity (84.1 µA/mM cm²) were obtained.
• The biosensor showed reliable performance in serum samples.

Conclusions:

• The developed Ti3C2Tx/Pt-Pd based sarcosine biosensor offers a promising tool for early prostate cancer diagnosis.
• The biosensor's high sensitivity and stability address limitations of current detection methods.
• This approach provides a valuable reference for detecting other cancer biomarkers.