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Raoultibacter phocaeensis sp. nov., A New Bacterium Isolated from a Patient with Recurrent Clostridioides difficile
Abdourahamane Yacouba1,2,3, Edmond Kuete Yimagou1,2, Cheikh Ibrahima Lo2,4
1Aix Marseille Univ, IRD, Microbes, Evolution, Phylogeny and Infection (MEPHI), AP-HM, Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.
Current Microbiology
|July 20, 2022
Summary
A new bacterial species, Raoultibacter phocaeensis, was identified from a patient with recurrent Clostridioides difficile infection. This discovery expands the Raoultibacter genus and offers insights into novel bacteria associated with C. difficile infections.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Infectious Diseases
Background:
- Clostridioides difficile infection (CDI) is a significant healthcare concern, often characterized by recurrent episodes.
- The gut microbiome plays a crucial role in CDI pathogenesis and recurrence.
Purpose of the Study:
- To characterize a novel bacterial species isolated from a patient experiencing recurrent CDI.
- To determine the taxonomic and phylogenetic placement of this new isolate within the genus Raoultibacter.
Main Methods:
- Phenotypic characterization including Gram staining, motility, and spore formation.
- Fatty acid methyl ester (FAME) profiling.
- Genomic analysis including genome size, G+C content, and gene prediction.
- Phylogenetic analysis based on 16S rRNA gene sequencing.
- Whole-genome-based phylogenetic analyses (OrthoANI and digital DNA-DNA hybridization).
Main Results:
- Strain Marseille-P8396T is a Gram-positive, nonspore-forming, nonmotile rod.
- Major fatty acids include 9-Octadecenoic acid (41.9%), hexadecanoic acid (22.5%), and 11-Octadecenoic acid (11.0%).
- Optimal growth occurs anaerobically at 37°C, pH 7.5, with a genome size of 3.86 Mb and 59.4% G+C content.
- Phylogenetic analysis revealed it as a new species within the Raoultibacter genus, named Raoultibacter phocaeensis sp. nov.
Conclusions:
- Strain Marseille-P8396T represents a novel species, Raoultibacter phocaeensis, distinct from its closest relative, Raoultibacter timonensis.
- This finding contributes to the understanding of bacterial diversity associated with C. difficile infections.
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