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Updated: Sep 4, 2025

A Rhodopsin Transport Assay by High-Content Imaging Analysis
Published on: January 16, 2019
Molecular Biology of Microbial Rhodopsins
1Department Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany. martin.engelhard@mpi-dortmund.mpg.de.
Abstract:
Research on type 1 rhodopsins spans now a history of 50 years. Originally, just archaeal ion pumps and sensors have been discovered. However, with modern genetic techniques and gene sequencing tools, more and more proteins were identified in all kingdoms of life. Spectroscopic and other biophysical studies revealed quite diverse functions. Ion pumps, sensors, and channels are imprinted in the same seven-helix transmembrane protein scaffold carrying a retinal prosthetic group. In this review, molecular biology methods are described, which enabled the elucidation of their function and structure leading to optogenetic applications.
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