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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

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A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo
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Optogenetic Control of Human Stem Cell-Derived Neurons.

Rouhollah Habibey1, Johannes Striebel1, Kritika Sharma1

  • 1Department of Ophthalmology, Universitäts-Augenklinik Bonn, University of Bonn, Bonn, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|July 20, 2022
PubMed
Summary

Researchers demonstrate a method to study human stem cell-derived neurons using optogenetics and electrophysiology. This technique allows for detailed analysis of neuronal activity at both single-cell and circuit levels.

Keywords:
Banker cultureInduced pluripotent stem cells (iPSCs)Long-term iPSC-derived neuronal cultureMulti-electrode array (MEA) electrophysiologyNeuron-astrocyte co-cultureOptogenetic stimulationPatch clamp

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Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Electrophysiology

Background:

  • Human induced pluripotent stem cell (hiPSC)-derived neurons are valuable models for studying neurological function and disease.
  • Characterizing the functional properties of these neurons is crucial for understanding neural circuits.
  • Existing electrophysiological methods can be enhanced with targeted stimulation techniques.

Purpose of the Study:

  • To present a comprehensive methodology for preparing and analyzing hiPSC-derived neurons using optogenetics.
  • To enable simultaneous functional recordings via patch clamp and multi-electrode array (MEA) electrophysiology.
  • To characterize spontaneous and evoked neuronal activity in hiPSC-derived neurons.

Main Methods:

  • Preparation of hiPSC-derived neurons engineered to express optogenetic actuators.
  • In vitro optogenetic stimulation protocols.
  • Simultaneous electrophysiological recordings using patch clamp and MEA.

Main Results:

  • Successful optogenetic activation of hiPSC-derived neurons.
  • Demonstration of simultaneous patch clamp and MEA recordings.
  • Characterization of both spontaneous and optogenetically evoked neuronal activity.

Conclusions:

  • The presented approach allows for detailed functional characterization of hiPSC-derived neurons.
  • Optogenetic stimulation combined with advanced electrophysiology provides insights into single neuron and circuit dynamics.
  • This methodology serves as a powerful tool for neuroscience research and drug discovery.