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Related Experiment Video

Updated: Sep 4, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

Expanding the epitranscriptome: Dihydrouridine in mRNA.

Sameer Dixit¹, Samie R Jaffrey¹

¹Department of Pharmacology, Weill-Cornell Medical College, Cornell University, New York, New York, United States of America.

|July 20, 2022

Summary

This study reveals that approximately 130 yeast messenger RNAs (mRNAs) contain dihydrouridine, a uridine derivative. Dihydrouridine can influence mRNA splicing, impacting gene expression regulation.

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Last Updated: Sep 4, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

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Published on: August 22, 2019

Area of Science:

Molecular Biology
RNA Metabolism

Background:

Nucleotide modifications play a crucial role in regulating mRNA processing and stability.
The presence and function of specific modified nucleosides in mRNA are increasingly recognized as important regulatory mechanisms.

Purpose of the Study:

To identify and characterize mRNAs containing dihydrouridine in yeast.
To investigate the functional impact of dihydrouridine on mRNA processing, specifically splicing.

Main Methods:

Bioinformatic analysis to identify potential dihydrouridine-modified mRNAs.
Experimental validation of dihydrouridine presence in selected yeast mRNAs.
Functional assays to assess the effect of dihydrouridine on mRNA splicing.

Main Results:

Approximately 130 yeast mRNAs were found to contain dihydrouridine.
Functional studies demonstrated that dihydrouridine can affect mRNA splicing in certain cases.

Conclusions:

Dihydrouridine is a prevalent modification in yeast mRNA.
This modification can directly influence mRNA splicing, suggesting a novel layer of gene expression regulation.