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Updated: Sep 4, 2025
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Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor
Published on: May 2, 2025
438
Towards 50 years of platelet function analyser (PFA) testing.
Emmanuel J Favaloro1,2,3, Leonardo Pasalic1,2,4, Giuseppe Lippi5
1Haematology, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead Hospital, Westmead, NSW, Australia.
Clinical Chemistry and Laboratory Medicine
|July 20, 2022
Summary
The platelet function analyzer (PFA) screens platelet function using closure time (CT) with two models, PFA-100 and PFA-200. It
Area of Science:
- Hematology
- Clinical Diagnostics
- Medical Devices
Background:
- The platelet function analyzer (PFA) is a widely used instrument for assessing platelet function.
- Two models exist: the PFA-100 and the PFA-200, both providing closure time (CT) measurements.
- Normal reference ranges are consistent across both models for specific test cartridges.
Approach:
- This review summarizes the historical development of the PFA system.
- It details the different test cartridges available: collagen/epinephrine (C/Epi), collagen/adenosine diphosphate (C/ADP), and Innovance PFA P2Y.
- The review discusses the sensitivity of the PFA for detecting von Willebrand disease (VWD) and the impact of aspirin therapy.
Key Points:
- The PFA demonstrates high sensitivity for von Willebrand disease (VWD) and aspirin effects.
- Sensitivity is moderate for general platelet function defects or count deficiencies.
- Recommendations for PFA use vary based on user expertise and clinical application.
Conclusions:
- The PFA-200 is intended to replace the PFA-100, though not yet available globally.
- The PFA system has applications in assessing thrombosis and pre-operative bleeding risks.
- This review provides an overview of PFA history and current clinical utility.
