Cell membrane water permeability of rabbit cortical collecting duct

The water permeability (Posm) of the cell membranes of isolated perfused rabbit cortical collecting ducts was measured by quantitative light microscopy. Water permeability of the basolateral membrane, corrected for surface area, was 66 microns X sec-1 for principal cells and 62.3 microns X sec-1 for intercalated cells. Apical membrane Posm values corrected for surface area, were 19.2 and 25 microns X sec-1 for principal and intercalated cells, respectively, in the absence of antidiuretic hormone (ADH). Principal and intercalated cells both responded to ADH by increasing Posm of their apical membranes to 92.2 and 86.2 microns X sec-1, respectively. The ratio of the total basolateral cell membrane osmotic water permeability to that of the apical cell membrane was approximately 27:1 in the absence of ADH and approximately 7:1 in the presence of the hormone for both cell types. This asymmetry in water permeability is most likely due to the fact that basolateral membrane surface area is at least 7 to 8 times greater than that of the apical membrane. Both cell types exhibited volume regulatory decrease when exposed to dilute serosal bathing solutions. Upon exposure to a hyperosmotic serosal bath (390 mosM), principal cells did not volume regulate while two physiologically distinct groups of intercalated cells were observed. One group of intercalated cells failed to volume regulate; the second group showed almost complete volume regulatory increase behavior.