Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering extracellular vesicles for targeted therapeutic delivery in the heart.

Bioscience reports·2026
Same author

Sustained β-catenin activation via GSK3 inhibition promotes direct fibroblast-to-cardiomyocyte reprogramming.

The Journal of biological chemistry·2026
Same author

Hemoglobin inhibits fibroblast-to-cardiomyocyte reprogramming via TLR2/TLR4-dependent chromatin compaction.

Molecular therapy. Nucleic acids·2026
Same author

Nucleosome repositioning in cardiac reprogramming.

PloS one·2025
Same author

Nucleosome repositioning in cardiac reprogramming.

bioRxiv : the preprint server for biology·2024
Same author

The impact of aging on cardiac repair and regeneration.

The Journal of biological chemistry·2024

Related Experiment Video

Updated: Sep 4, 2025

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
05:04

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Published on: February 22, 2022

3.5K

Conservation of miR combo based direct cardiac reprogramming.

Syeda Samara Baksh1, Conrad P Hodgkinson1

  • 1Mandel Center for Heart and Vascular Research, and the Duke Cardiovascular Research Center, Duke University Medical Center, Durham, NC, 27710, USA.

Biochemistry and Biophysics Reports
|July 21, 2022
PubMed
Summary

Cardiac fibroblast reprogramming using a microRNA combination (miR combo) effectively converts cells into cardiomyocyte-like cells across multiple mammalian species. This approach bypasses species-specific limitations seen with transcription factors.

Keywords:
CardiomyocytesFibroblastsReprogrammingmiRNAs

More Related Videos

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.2K
Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
10:05

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

8.8K

Related Experiment Videos

Last Updated: Sep 4, 2025

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
05:04

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Published on: February 22, 2022

3.5K
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.2K
Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
10:05

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

8.8K

Area of Science:

  • Cardiovascular Biology
  • Regenerative Medicine
  • Molecular Cardiology

Background:

  • Direct cardiac reprogramming offers a promising avenue for heart regeneration by converting resident fibroblasts into cardiomyocytes.
  • Transcription factor-based reprogramming strategies exhibit species-specific efficacy, limiting their translational potential.
  • The species-specificity of microRNA (miRNA)-based cardiac reprogramming remains largely unexplored.

Purpose of the Study:

  • To investigate the species-specificity of a defined four-miRNA combination (miR combo) for direct cardiac fibroblast reprogramming.
  • To determine if miR combo can effectively reprogram cardiac fibroblasts from different mammalian species into cardiomyocyte-like cells.
  • To compare the efficacy of miRNA-based reprogramming with existing transcription factor-based methods.

Main Methods:

  • Isolation of cardiac fibroblasts from the left ventricles of dogs, pigs, and humans.
  • Transfection of these fibroblasts with a specific four-miRNA combination (miR combo: miR-1, miR-133a, miR-208a, miR-499).
  • Quantitative PCR (QPCR) to assess gene expression changes and Actinin-2 (Actn2) staining to identify cardiomyocyte-like cells.

Main Results:

  • miR combo successfully reprogrammed cardiac fibroblasts from dogs, pigs, and humans.
  • Significant upregulation of cardiac developmental genes, sarcomere-associated genes, and cardiac ion channel genes was observed across all species.
  • Actn2+ staining confirmed the successful induction of cardiomyocyte-like cell morphology in reprogrammed fibroblasts from all tested species.

Conclusions:

  • The studied miRNA combination (miR combo) effectively reprograms mammalian cardiac fibroblasts into cardiomyocyte-like cells, demonstrating broad species applicability.
  • Unlike transcription factor-based methods, miR combo-mediated reprogramming overcomes species-specific limitations.
  • This finding supports the potential of miR combo as a universal strategy for cardiac regeneration.