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Updated: Sep 4, 2025

Preparation of Viral DNA from Nucleocapsids
Published on: August 16, 2011
Packaging of DNA origami in viral capsids: towards synthetic viruses
Stanislav Kler1, Ran Zalk2, Alexander Upcher2
1Geza Ad Ltd, Givatayim 53251, Israel. slavakler1@gmail.com.
Abstract:
We report a new type of nanoparticle, consisting of a nucleic acid core (>7500 nt) folded into a 35 nm DNA origami sphere, encapsulated by a capsid composed of all three SV40 virus capsid proteins. Compared to the prototype reported previously, whose capsid consists of VP1 only, the new nanoparticle closely adopts the unique intracellular pathway of the native SV40, suggesting that the proteins of the synthetic capsid retain their native viral functionality. Some of the challenges in the design of such near-future composite drugs destined for gene delivery are discussed.
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