Related Experiment Video

Updated: Sep 4, 2025

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10:33

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

10.9K

First Description of a Yersinia pseudotuberculosis Clonal Outbreak in France, Confirmed Using a New Core Genome

Cyril Savin1,2,3, Anne-Sophie Le Guern1,2,3, Fanny Chereau4

  • 1Institut Pasteurgrid.428999.7, Université de Paris Cité, Yersinia Research Unit, Paris, France.

Microbiology Spectrum
|July 21, 2022
PubMed

Insights

A new core-genome multilocus sequence typing (cgMLST) method identified France's first Yersinia pseudotuberculosis clonal outbreak, linked to tomatoes. This real-time molecular typing supports investigations into foodborne pathogen outbreaks.

Area of Science:

  • Microbiology
  • Epidemiology
  • Food Safety

Background:

  • Yersinia pseudotuberculosis is an enteric pathogen causing various illnesses, with sporadic cases and documented outbreaks.
  • Outbreaks are often linked to contaminated food sources, necessitating robust surveillance and typing methods.

Purpose of the Study:

  • To report the first Yersinia pseudotuberculosis clonal outbreak in France (2020).
  • To introduce and validate a novel core-genome multilocus sequence typing (cgMLST) scheme for real-time molecular typing of Y. pseudotuberculosis.

Main Methods:

  • Development of a new cgMLST scheme with 1,921 genes specific to Y. pseudotuberculosis.
  • Application of the cgMLST scheme for epidemiological investigation and molecular typing of outbreak isolates.
  • Retrospective analysis of earlier isolates using the developed cgMLST method.

Main Results:

  • Identification of a Y. pseudotuberculosis clonal outbreak in France during summer 2020, with tomatoes as the suspected source.
  • The cgMLST scheme successfully clustered outbreak isolates and enabled real-time molecular typing.
  • Retrospective analysis identified additional cluster isolates from earlier in 2020.

Conclusions:

  • The novel cgMLST method provides excellent discriminatory power for Y. pseudotuberculosis typing.
  • This user-friendly method supports real-time identification and investigation of clonal outbreaks.
  • The method is now systematically used at the Yersinia National Reference Laboratory and available to the scientific community.
Keywords:
Yersinia pseudotuberculosiscgMLSTenteric yersiniosisepidemiological investigationoutbreak

More Related Videos

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

8.7K
'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens11:31

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Published on: July 8, 2011

13.6K

Related Concept Videos

Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
63.0K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
119
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
71.7K
Steps in Outbreak Investigation01:18

Steps in Outbreak Investigation

In the ever-evolving field of public health, statistical analysis serves as a cornerstone for understanding and managing disease outbreaks. By leveraging various statistical tools, health professionals can predict potential outbreaks, analyze ongoing situations, and devise effective responses to mitigate impact. For that to happen, there are a few possible stages of the analysis:
179
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
127
Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
178