Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

4.9K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.9K
MicroRNAs01:22

MicroRNAs

3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
Overview of Exosomes01:36

Overview of Exosomes

2.8K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Independent and interactive effects of personal light exposure and air pollution on incident COPD: A prospective cohort study with 13 million hours of light sensor data.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Understanding mechanisms of combined polystyrene and cadmium toxicity in <i>Leonurus japonicus</i> Houtt. across varying pH levels.

RSC advances·2026
Same author

EAT-Lancet Diet, Plasma Metabolites, and Risk of Peripheral Artery Disease: A Prospective Cohort Study.

Journal of the American Heart Association·2026
Same author

Conjugated Polymer Semiconductors Enabled Multifunctional Interfacial Engineering for High-Performance Inverted Perovskite Solar Cells.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Astaxanthin ameliorates cardiac damage induced by prepubertal di(2-ethylhexyl) phthalate exposure via inhibition of PPAR-α-mediated excessive autophagy.

Journal of environmental sciences (China)·2026
Same author

Enhancing Efficiency and Stability of Perovskite Solar Cells Through Electron-Rich Covalent Organic Frameworks Radicals.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: Sep 4, 2025

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
08:49

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

11.2K

Exosomes from bone marrow mesenchymal stem cells decrease chemosensitivity of acute myeloid leukemia cells via

Juan Wu1, Yaqin Zhang2, Xiaoyu Li2

  • 1Department of Laboratory Medicine, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou, 350004, PR China.

Biochemical and Biophysical Research Communications
|July 21, 2022
PubMed
Summary

Bone marrow mesenchymal stem cells (BMSCs) release exosomes containing miR-10a, which protect acute myeloid leukemia (AML) cells from chemotherapy. This communication pathway enhances AML cell survival and chemoresistance by targeting RPRD1A and activating Wnt/β-catenin signaling.

Keywords:
Acute myeloid leukemiaBone mesenchymal stem cellsChemosensitivityExosomesmiR-10a

More Related Videos

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

1.3K
Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
10:55

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

8.3K

Related Experiment Videos

Last Updated: Sep 4, 2025

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
08:49

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

11.2K
Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

1.3K
Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
10:55

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

8.3K

Area of Science:

  • Cancer Biology
  • Cellular Communication
  • Stem Cell Research

Background:

  • Bone marrow mesenchymal stem cells (BMSCs) are crucial components of the acute myeloid leukemia (AML) microenvironment.
  • BMSCs influence AML progression and treatment resistance.

Purpose of the Study:

  • To investigate the role of exosome-mediated communication between BMSCs and AML cells.
  • To elucidate the specific molecular mechanisms by which BMSCs affect AML cell chemosensitivity.

Main Methods:

  • Co-culture of AML cells with BMSC-derived exosomes (BMSCs-Exos).
  • Quantification of miR-10a levels in exosomes and AML cells.
  • Assessment of AML cell chemosensitivity to cytarabine.
  • Analysis of RPRD1A expression and Wnt/β-catenin pathway activation.

Main Results:

  • BMSCs-Exos reduced AML cell sensitivity to cytarabine.
  • miR-10a was significantly elevated in exosomes from AML-BMSCs and upregulated in AML cells post-co-culture.
  • Upregulated miR-10a conferred chemoresistance by downregulating RPRD1A and activating Wnt/β-catenin signaling.
  • Downregulating miR-10a restored chemosensitivity in AML cells treated with BMSCs-Exos.

Conclusions:

  • AML-BMSCs deliver miR-10a via exosomes to AML cells, promoting chemoresistance.
  • The miR-10a/RPRD1A/Wnt/β-catenin axis represents a novel therapeutic target for overcoming AML treatment resistance.