One-step high-throughput detection of low-abundance biomarker BDNF using a biolayer interferometry-based 3D...

Area of Science:

• Biomedical Engineering
• Biosensor Technology
• Ophthalmology

Background:

• Early detection of glaucoma is crucial for preventing irreversible blindness.
• Current high-throughput detection methods for low-abundance biomarkers like BDNF are challenging.
• Biosensors offer potential for sensitive and rapid disease screening.

Purpose of the Study:

• To develop a one-step, high-throughput 3D aptasensor for sensitive detection of brain-derived neurotrophic factor (BDNF).
• To evaluate the aptasensor's utility for early glaucoma screening.
• To demonstrate a novel approach for low-abundance biomarker detection.

Main Methods:

• Constructed a dimeric aptamer for high-affinity BDNF targeting.
• Integrated highly sensitive 3D matrix sensors for signal transduction.
• Utilized biolayer interferometry (BLI) Octet system for automated, real-time monitoring.

Main Results:

• Achieved a broad BDNF detection window (0.41–250 ng/mL) with a low limit of detection (0.2 ng/mL).
• Demonstrated one-step batch conversion of BDNF binding to optical signals.
• Showed good agreement between aptasensor results, ELISA, and clinical diagnosis in glaucoma patients.

Conclusions:

• The developed 3D aptasensor enables sensitive and rapid detection of BDNF for early glaucoma screening.
• This one-step, high-throughput approach is a valuable tool for glaucoma diagnosis.
• The system has the potential to reduce blindness by facilitating early intervention.