An overview of synthesis, characterization, applications and associated adverse effects of bioactive nanoparticles

A particle with a diameter ranging from 1 to 100 nm is considered a nanoparticle (NP). Owing to their small size and high surface area, NPs possess unique physical, chemical and biological properties as compared to their bulkier counterparts. This paper describes various physico-chemical as well as green methods that can be used to synthesize different types of NPs including carbon-based, ceramic, metal, semiconductor, polymeric and lipid-based NPs. These methods can be categorized into either top-down or bottom-up approaches. Electron microscopy, atomic force microscopy, dynamic light scattering, X-ray diffraction, zeta-potential instrument, liquid chromatography-mass spectrometry, fourier transform infrared spectroscopy and thermogravimetric analysis are the techniques discussed in the characterization of NPs. This review provides an insight into the extraordinary properties of NPs that have opened the doors for endless biomedical applications like drug delivery, photo-ablation therapy, biosensors, bio-imaging and hyperthermia. In addition, NPs are also involved in improving crop growth, making protective clothing, cosmetics and energy reserves. This review also specifies adverse health effects associated with NPs such as hepatotoxicity, genotoxicity, neurotoxicity, etc., and inhibitory effects on plant growth and aquatic life. Further, in-vitro toxicity assessment assays for cell proliferation, apoptosis, necrosis and oxidative stress, as well as in-vivo toxicity assessment like biodistribution, clearance, hematological, serological and histological studies, are discussed here. Lastly, the authors have mentioned various measures that can be adopted to minimize the toxicity associated with NPs such as green synthesis, use of stabilizers, gene gun, polymer shell, microneedle capsule, etc.