Thermal stress and TiO₂ nanoparticle-induced oxidative DNA damage and apoptosis in mouse hippocampus

Titanium dioxide (nano-TiO₂) is used abundantly in various industrial products and novel medical therapies. In addition, the impact of climate change on the health and safety will undoubtedly increase in the future. However, the effects of exposure to these nanoparticles and heat stress on hippocampal DNA damage and apoptosis remain unclear. This study was conducted to evaluate the DNA damage and apoptosis in the hippocampal tissue and the physiological responses in mice induced by intraperitoneal (i.p.) administration of TiO₂ nanoparticles (NPs) and heat stress for 14 consecutive days. The results showed that heat stress and TiO₂-NPs were induced in the mouse hippocampus that led to hippocampal reactive oxygen species generation, oxidative damage of DNA, and apoptosis in a partly dose-dependent manner, especially at very hot temperature. High doses of nanosized TiO₂ and severe heat stress significantly damaged the function of the hippocampus, as shown in the comet assay and apoptosis tests. The results of this study may provide data for appropriate measures to control and assess the risk of nano-TiO₂ and thermal stress hazards to human health, especially workers. Safety guidelines and policies should be considered when handling nanomaterials in a hot environment.