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Related Experiment Video

Updated: Sep 4, 2025

Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond

Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond

Published on: June 24, 2015

Real-Time Image Processing Toolbox for All-Optical Closed-Loop Control of Neuronal Activities.

Weihao Sheng¹, Xueyang Zhao¹, Xinrui Huang¹

¹School of Life Sciences and Technology, ShanghaiTech University, Shanghai, China.

Frontiers in Cellular Neuroscience

|July 22, 2022

Summary

Researchers developed ORCA software for fast analysis of neuronal imaging data. This enables real-time, all-optical control of neural circuits, advancing neuroscience research.

Keywords:

calcium imaging closed-loop optogenetic stimulation fast image processing image alignment image registration neuron segmentation

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Last Updated: Sep 4, 2025

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Published on: June 24, 2015

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

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Published on: September 2, 2013

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

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Published on: September 16, 2022

Area of Science:

Neuroscience
Computational Biology
Biotechnology

Background:

All-optical control of neural circuits is advancing rapidly.
Current applications are limited by the lack of efficient software for online analysis of neuronal imaging data.

Purpose of the Study:

To develop a software solution for real-time analysis of neuronal imaging data.
To enable all-optical, closed-loop control of neural circuit activity.

Main Methods:

Developed ORCA (Online Real-time activity and offline Cross-session Analysis) software.
Implemented image registration, neuron segmentation, and activity extraction exceeding 100 frames per second.
Utilized a purely statistical algorithm for active neuron detection.

Main Results:

ORCA achieves high-speed analysis, supporting real-time neural activity detection and readout.
Demonstrated successful closed-loop control of neurons identified on the fly.
Included a cross-session alignment module for tracking neurons across multiple experimental sessions.

Conclusions:

ORCA is a powerful toolbox for rapid analysis of imaging data.
Provides a solution for all-optical closed-loop control of neuronal activity, enhancing neuroscience research capabilities.