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Updated: Sep 4, 2025
High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
Published on: February 1, 2011
Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and
Zhenhao Zhang1,2, Tong He1,2, Yan Qi1
1Shaanxi Key Laboratory of Brain Disorders, Shaanxi Provincial Key Laboratory of Infection and Immunity Diseases, Institute of Basic and Translational Medicine, Xi'an Medical University No. 1 Xin Wang Road Xi'an 710021 China gouxingchun@189.cn +86 29 86177603.
Abstract:
We have developed a rapid and specific method for site-specific 5-hydroxymethylcytosine (5hmC) quantification at single-base resolution. This bisulfite-free method integrates the peroxotungstate oxidization with the mismatched probe-assisted ligation to guarantee the specificity. Moreover, the high-efficiency LAMP also makes the proposed method suitable for the detection of low-content samples.
