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Serological relationship between mouse adenovirus strains FL and K87
Laboratory Animal Science
|February 1, 1987
Summary
Mouse adenovirus infection diagnosis requires using both FL and K87 viral antigens. Serum from mice infected with the FL strain cross-reacts with K87, but K87 infection serum only reacts with K87 antigen.
Area of Science:
- Veterinary Virology
- Immunology
- Mouse Models
Background:
- Mouse adenovirus (MAV) is a common pathogen in laboratory mice.
- Accurate serologic diagnosis is crucial for managing MAV infections in research colonies.
Purpose of the Study:
- To investigate the antigenic relationship between FL and K87 strains of mouse adenovirus.
- To evaluate the efficacy of complement fixation and indirect immunofluorescence tests for MAV diagnosis.
Main Methods:
- Outbred mice were inoculated with FL or K87 MAV strains via intraperitoneal, oral, or intranasal routes.
- Serum samples were collected at intervals post-infection.
- Complement fixation and indirect immunofluorescence assays were performed using FL and K87 antigens.
Main Results:
- A unilateral antigenic relationship was observed between FL and K87 strains.
- Serum from FL-infected mice cross-reacted with both FL and K87 antigens.
- Serum from K87-infected mice reacted only with the homologous K87 antigen.
- Indirect immunofluorescence generally yielded higher antibody titers than complement fixation.
Conclusions:
- Specific serologic diagnosis of MAV infection necessitates the use of both FL and K87 antigens.
- The observed cross-reactivity impacts diagnostic test design and interpretation.
- Indirect immunofluorescence may offer increased sensitivity for detecting MAV antibodies.