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Capturing Chromosome Conformation Across Length Scales
Published on: January 20, 2023
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Targeted Chromosome Conformation Capture (HiCap)
Artemy Zhigulev1, Pelin Sahlén2
1Department of Gene Technology, Science for Life Laboratory, Royal Institute of Technology, Stockholm, Sweden.
Methods in Molecular Biology (Clifton, N.J.)
|July 22, 2022
Summary
Targeted chromosome conformation capture (HiCap) enables detection of genomic feature interactions. This method combines Hi-C with sequence capture technology for precise analysis.
Area of Science:
- Genomics
- Molecular Biology
- Biotechnology
Background:
- Understanding 3D genome organization is crucial for deciphering gene regulation.
- Existing methods may lack specificity or efficiency in detecting long-range interactions.
Purpose of the Study:
- To describe the Targeted Chromosome Conformation Capture (HiCap) experimental method.
- To detail the protocol for designing capture probes and performing Hi-C.
- To explain the integration of Hi-C with sequence capture technology.
Main Methods:
- Design of sequence capture probes targeting specific genomic regions.
- Adaptation of Chromosome Conformation Capture (Hi-C) for next-generation sequencing.
- Coupling Hi-C with sequence capture technology for targeted interaction detection.
Main Results:
- A detailed protocol for HiCap is presented.
- The methodology allows for focused analysis of specific genomic interactions.
- Successful integration of sequence capture with Hi-C is achieved.
Conclusions:
- HiCap provides a powerful approach for studying spatial genomic interactions.
- This method enhances the specificity of chromosome conformation capture.
- The described protocol facilitates targeted investigation of promoter-enhancer interactions.
