Related Experiment Video

Updated: Sep 4, 2025

Using a Pan-Viral Microarray Assay Virochip to Screen Clinical Samples for Viral Pathogens
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Novel Human Parechovirus 3 Diversity, Recombination, and Clinical Impact Across 7 Years: An Australian Story

Seweryn Bialasiewicz1,2, Meryta May2,3, Sarah Tozer2

  • 1School of Chemistry and Molecular Biosciences, The University of Queensland, Australian Centre for Ecogenomics, St Lucia, Australia.

The Journal of Infectious Diseases
|July 22, 2022
PubMed
Abstract

Insights

A new human parechovirus 3 Australian recombinant (HPeV3-AR) strain emerged in 2013, causing infant sepsis outbreaks. This study found HPeV3-AR evolved but was not linked to increased infant illness severity.

Area of Science:

  • Virology
  • Molecular Evolution
  • Infectious Diseases

Background:

  • A novel human parechovirus 3 Australian recombinant (HPeV3-AR) strain emerged in 2013.
  • This emergence coincided with biennial outbreaks of sepsis-like illnesses in infants.

Purpose of the Study:

  • To evaluate the molecular evolution of the HPeV3-AR strain.
  • To assess the association between the HPeV3-AR strain and severe human parechovirus infections in infants.

Main Methods:

  • Sequencing of HPeV3-positive samples from hospitalized infants (2013-2020) and a community birth cohort (2010-2014).
  • Phylogenetic and evolutionary analyses of coding regions.
  • Development and application of a recombinant-specific PCR for screening.

Main Results:

  • The HPeV3-AR strain showed progressive evolution, particularly in nonstructural genes.
  • HPeV3-AR was detected in hospitalized infants from late 2013 onwards, dominating early outbreaks.
  • The AR strain was not statistically associated with increased clinical severity in hospitalized infants.

Conclusions:

  • HPeV3-AR was the dominant strain during the study period.
  • Increased hospital admissions may be attributed to a temporary fitness advantage and/or increased virulence of the HPeV3-AR strain.
Keywords:
AustraliaHPeV3communityinfantsparechoviruspediatricpicornavirusrecombinationsepsisviral evolution

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