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Wheat transcriptomic responses to extended feeding by wheat curl mites
Lise Pingault1, Tran Kim Ngan Luong1, Joe Louis1,2
1Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Scientific Reports
|July 22, 2022
Summary
Wheat curl mite (WCM) feeding significantly alters wheat gene expression, revealing defense mechanisms in resistant and susceptible cultivars after 10 days. This study enhances understanding of wheat
Area of Science:
- Plant Science
- Entomology
- Genomics
Background:
- Wheat production faces significant yield losses due to pests, notably the wheat curl mite (WCM).
- WCM infestation causes leaf curling and transmits viruses, impacting wheat health.
- Limited knowledge exists on WCM's long-term effects on the wheat transcriptome.
Purpose of the Study:
- To investigate wheat transcriptome variations following extended WCM feeding (10 days post-infestation).
- To compare transcriptomic responses between WCM-resistant (Byrd) and WCM-susceptible (Settler CL) wheat cultivars.
Main Methods:
- RNA-sequencing (RNA-seq) was employed to analyze gene expression.
- WCM-infested and uninfested wheat leaves were collected at 10 days post-infestation.
- Comparative transcriptomic analysis was performed on two distinct wheat cultivars.
Main Results:
- Identified common and specific transcriptomic variations between resistant and susceptible wheat cultivars.
- Localized differentially expressed genes to chromosome 3D, implicating defense and stress response functions.
- Discovered gene functions related to lipid signaling, membrane integrity, and phytohormone pathways.
Conclusions:
- Extended WCM feeding induces complex transcriptomic changes in wheat.
- Specific gene pathways related to defense and stress response are activated.
- Findings provide insights into wheat's defense mechanisms against WCM, crucial for crop improvement.
